Plant dna isolation pdf

Lei, Y. Gao, T. Tsering, S. Shi and Y. Zhong, mini-preparation of genomic DNA from needles of Determination of genetic variation in silver fir Abies alba Mill.

Mountain Region, China using inter-simple Dellaporta, S. Wood and J. Hicks, A sequence repeats. Plant Molecular Sao Paulo, 2.

Biology Report, 1: Chen, X. Yang, Species - diversified plant Reichardt, M. Rogers, In Current Protocols in climatic stress and soil erosion in subtropical hillside.

Molecular Biology, Eds. Ausubel, F. Kingston, D. Moore, J. Seidman, Smith and K. Nan, P. Shi, S. Peng, C. Tian and Y. Zhong, Supplement: Genetic diversity in Primula obconica Wang, Y. Taylor, Annals of Botany, fingerprinting and other PCR applications. Biotechniques, Muluvi, G.

Sprent, N. Soranzo, J. Provan, Khanuja, S. Shasany, M. Darokar and D. Odee, G. Folkard, J. McNicol, J. Provan S. Kumar, Powell, Plants Moringa. Molecular Ecology, 8: Molecular Biology Report, 17 1 : Cardoso, S. Eloy, J. Provan, M. Cardoso Keb Llanes, M. Gonzalez, B. Chi-Manzanero and and P. Ferreira, Genetic differentiation of D. Infante, Molecular Ecology, 9: Plants Molecular Biology Report 6. Sghaier, Z.

Mohammed, Genomic 20 3 : ae. DNA extraction method from pearl millet Pennisetum Arinathan, V. Mohan and A. De Britto, African Journal of Biotechnology, Chemical composition of certain tribal pulses in south 4 8 : Food Sci. Chakraborti, D. Sarkar, S. Gupta and S. Das, Fang, G. Hammar and R. Rebecca, A Small and large scale genomic DNA isolation quick and inexpensive method for removing protocol for chickpea Cicer arietinum suitable for polysaccharides from plant genomic DNA.

African J. Lodhi, M. Ye, N. Weeden and B. Reisch, Krasova-Wade, T. Neyra, Optimization Letters in extraction from grapevine cultivars and Vitis species applied microbiology, Plant Molecular Biology Report, Loomis, M. Overcoming problems of Yoon, C. Glawe, Pretreatment with enzymes and organelles. Howland, D. Oliver and A. Davy, Mejjard, M. Vedel and G. Ducreux, Plants Paterson, A. Brubaker and J. Wendel, Molecular Biology Report, A rapid method for extraction of cotton Gossypium Pich, U.

Schubert, Nucleic Acids Research, Honeycutt, R. Sorbal, P. Keim and J. Irvine, Dowling, T. Moritz, J. Palmer and A rapid DNA extraction method for sugarcane L. Rieseberg, Plant Molecular Biology Report, fragments and restriction sites. In Molecular Systematic, Eds. Hillis, D. Moritz and B. Sul, I. Korban, A highly efficient Mable, Sinauer, Sunderland, Mass, pp: Welsh, J. McClelland, Fingerprinting tissues. Nucleic 2: Acid Research, Willmitzer, L. Wagner, The isolation of Williams, J.

Kubelik, K. Livak, nuclei from tissue-cultured plant cells. Experimental J. Rafalski and S. Tingey, FW was obtained. Fig 1. DNA isolated from leaves of four plant spe- cies both young and old leaves, respectively re- solved on 0. The absorbances at , and nm were within the recommended ranges Roger and Bendih ; Doyle and Doyle LiCl was used as described by Pirttila et al. The Li-RNA complex is insoluble and thus gets efficiently precipitated, except for molecules smaller than bases.

In our study a little amount of RNA appr. In addition, LiCl precipi- tates also selectively shared DNA, residual proteins and neutral polysaccharides. The removal of RNA by LiCl was better facilitated by keeping the mixture in cold for some time, even though several workers have indicated that there is no effect of cold treatment on RNA precipitation with LiCl.

However, it was found advantageous that while using SDS along with a salt in DNA isolation, cold treatment should be avoided. LiCl is more powerful than NaCl in salting out the polysaccharides. Addition of BSA has many- fold benefits. It is known that BSA is a polyphenol absorbent Couch and Fritz, and it prevents denaturation of proteins. In addition, BSA is useful as it binds a wide range of inhibitory complexes that are carried over during the DNA extraction proce- Fig 2.

Fig 3. Restriction digestion of DNA isolated from four species young and old leaves, respectively with the restriction endonuclease Hind III along with their controls nondigested and digested, respectively. We tested several BSA concentrations within the range 0. However, in the present study, successful amplification Fig. Similar to the restriction digestion Fig. We also used like Khanuja et al. Using the present protocol we obtained high quality DNA with fairly good amount even in older leaves, which are otherwise thought to be recalcitrant for DNA isolation.

This protocol can probably be extended also to other angiosperm species. References Abdulova, G. Ananiev, P. Grozdanov, Plant Physiol. Anwander, E. Probst, B. Rode, Baier S. Mc Clements, The effect of glycerol, trehlose, sucrose and sorbitol on the thermostability of Bovine serum albumin. Food Colloids and Biopolymer Lab. Amherst, MA Buldewo S. Jaufeerally-Fakim, Couch J. Fritz, Isolation of DNA from plant high in polyphenolics.

Dellaporta S. Wood, J. Hicks, Doyle J. Doyle, Isolation of plant DNA from fresh tissue. Avalos, R. Fernandez-Martin, E. Cerda-Olmedo, E. Carlos, C. Domenech, Lovastatin inhibits the production of gibberellins but not sterol or carotenoid biosynthesis in Gibberella fujikuroi. Kaufman B. Richards, D. Dierig, DNA isolation method for high polysaccharide Lesquerella Species. Khanuja S. Shasany, M. Darokar, S. Kumar, Rapid isolation of DNA from dry and fresh samples of plants producing large amounts of secondary metabolites and essential oils.

Kreader C. Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein. Lee T. Timasheff, The stabilization of proteins by sucrose. Manning K. Isolation of nucleic acids from plants by differential solvent precipitation.

Ounzar, B. Hartmann, A. Rode, A. Benslimane, Date palm DNA minipreparation without liquid nitrogen. Pandey R. Adams, L. Flournoy, Pirttla A. Hirsikorpi, T. Kamarainen, L. Jaakola, A. Hohtola, DNA isolation method for Medicinal and aromatic plants. Raina K. Chandlee, Recovery of genomic DNA from fungus Sclerotinia homoeocarpa with high polysaccharide content. Rogers S. Bendich, Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Sambrook J.

Fritsch, T. Maniatis, Molecular Cloning A laboratory Manual. Cold Spring Harbor Laboratory Press. Sul I. Korban, A highly efficient method for isolating genomic DNA from plant tissues.

Plant Tissue Culture Biotech. Weishing K. Nybom, K. Wolff, W. Meyer,